![]() Īs a typical example, from a calibration plot following a linear equation taken here as the simplest possible model:į ( x ) = a x + b is the known standard deviation for the reagent blank's signal. Another consideration that affects the detection limit is the adequacy and the accuracy of the model used to predict concentration from the raw analytical signal. 3.2 being the most accepted value for this arbitrary value). The detection limit is estimated from the mean of the blank, the standard deviation of the blank, the slope ( analytical sensitivity) of the calibration plot and a defined confidence factor (e.g. ![]() In analytical chemistry, the detection limit, lower limit of detection, or LOD (limit of detection), often mistakenly confused with the analytical sensitivity, is the lowest quantity of a substance that can be distinguished from the absence of that substance (a blank value) with a stated confidence level (generally 99%).
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